BioSpectrum® 2D Imaging System

Main Features

• Capture superior images with the 8.3 megapixel high resolution, high sensitivity cooled CCD camera
• Motorized optics for setting the aperture, zoom and focus
• Automated templates for repeat experiments
• Light tight darkroom creates optimum imaging conditions
• Unique viewing window is UV blocking for quick sample inspection
• Built-in overhead white light and UV are easily selected from the software interface
• Automated control of the five-position emission filter array
• Easy access fold-down white light plate
• Chemi tray fits over the plate for placement of blots
• Roll-out tray provides easy access to the UV transilluminator
• Select from several benchtop, patented and awarding winning transilluminator models (configured with system or optional)
• VisionWorksLS Software (configured with system or optional)
• BioLite ready
   

Prodigy SameSpots 2D Analysis Software

• See Prodigy SameSpots tab (above)

• Automates your research for easy, yet accurate and repeatable results
• Controls the motorized zoom lens, epi and transillumination UV and white light, emission filter selection and system power
• Allows selection of user-defined or default settings
• Select identify new filters or select from SYBR Green (515-570mm), SYBR Gold (485-655mm), EtBr (570-640mm), clear and empty positions
• Click on the epi and transillumination light sources
• Set templates and user preferences for recurring experiments

Transillumination

• The white light plate folds down for illuminating samples such as coomassie blue stained or silver stained media
• Operate the white light via the software interface
• Flip the plate back when not in use
• To use the transilluminator, slide the transilluminator out on the sliding tray and place samples on the filter surface
• To fluoresce samples in the 460-470mm range, place the optional Visi-Blue Plate over the transilluminator surface

VisionWorksLS Image Acquisition and Analysis Software

VisionWorksLS Image Acquisition and Analysis Software can be configured with the system or ordered separately. Check with your local representative for details. This sophisticated package provides comprehensive tools for image capture and analysis of gels, plates and membranes as well as colony counting. Capabilities include:

• Image acquisition functions for simple image capture and expanded integration options
• Apply numerous image enhancements to images
• Comprehensive tools for advanced 1D lane densitometry and analysis including area density, molecular weights and colony counting
• Support for 21 CFR Part 11 compliance
• One-touch automated macros
• Define user profiles and preferences
• Generate extensive reports and export data
• Multiple user network license available
• More …
   

Specifications

• MegaCam 800 Camera
• Darkroom:
  • Highly uniform white light (<5% CV) for coomassie blue and silver stains
  • Light tight
  • Wide access door with UV safety shutoff
  • Gel viewing window
  • Sliding tray for transilluminator
  • Epi white and UV illumination (365nn and 480nm)
• Emission Filters - five positions:
  • SYBR Green 515-570nm
  • SYBR Gold 485-655nm
  • EtBr Red 570-640nm
• Software:
  • Interface for automated controls
  • VisionWorksLS (system may be configured with software or optional, check with your UVP representative)
  • Windows 2000 SP4, Windows XP Pro SP2  
• Transilluminators: Select from FirstLight or Benchtop models (system may be configured with software or is optional, check with your UVP representative)

FirstLight UV Transilluminator offers extremely uniform UV (<5% CV) which is important for capturing consistent 2D gel.

Options

• Cameras
• BioLite MultiSpectral Source: (optional)
• Fiber Optic Epi-Illumination
• Fiber Optic Transillumination
• VisionWorksLS Analysis Software: Image acquisition plus extensive enhancement and analysis capabilities
• Transilluminators: Select from the FirstLight or Benchtop models
• Converter Plates:
  • Visi-Blue Converter Plate
  • Longwave Converter Plate
• Thermal Printer: For archive quality prints in seconds
• Gel-Tools:
  • Gel-Sentry
  • Step Tablet
  • Gel-Cutter
  • Gel-Ruler
  • Gel-Scooper
  • Gel-Trays
• Glowell Luminescent Standard
• Computer and monitor
• Flat Field Correction (FFC) Plate for Microtiter Plates

• Download Brochure

A guided workflow for straightforward 2D analysis. Get rapid results from your proteomics data...

Prodigy SameSpots 2D Software from Nonlinear Dynamics is what users need to get fast results from 2D gels. It offers an economical solution to 2D analysis problems commonly faced by occasional or low throughput users, such as lack of reproducibility, labor intensive protocols and low confidence in results. Prodigy gives the benefits of speed and objectivity with a guided approach. Get the answers needed using an optimized workflow to quickly find significant proteins for subsequent picking and reporting.

• Simplicity.   A simplified workflow guides the user through each analysis step allowing effortless identification of significantly changing proteins.
• Objectivity.   The guided workflow promotes a consistent analysis approach allowing new or inexperienced users to generate completely objective and reproducible results from their 2D gels.
• Speed.   Analysis times of less than 10 minutes per image* are typical due to the high streamlined and optimized workflow.

Unique SameSpots Approach

SameSpots is a unique and validated approach delivering a major advance for the analysis of single or multiple dyes. At the foundation of the SameSpots approach is highly accurate technology which makes pixel perfect alignment of images achievable. This produces 100% matching of spots across all images and no missing values in your analyzed data. Your results are statistically robust and you can have absolute confidence in the data generated.

Performing spot detection on perfectly aligned images also means that spot editing is not required on most experiments, so operator bias can be kept to an absolute minimum. The result is an incredibly rapid analysis so you can quickly identify the significant proteins with negligible effort.

Key Steps in the Workflow

Image Quality Control

• Automatic image quality control assessment and feedback at the start of your workflow highlights many potential issues with the image quality before you proceed to the next step

Image Alignment

• Advanced image alignment guides you through the process of adding vector landmarks in a logical manner across your gel image
• Automatic alignment process uses the landmarks you set up to fully align all experimental images

Image Alignment Review

• Alignment grid draws attention to problematic areas on the gel image which are most distorted, so during the systematic alignment review you can focus on those areas
• Alignment can be manually adjusted if required

Prefiltering and SameSpots Analysis

• Artifacts which you do not want to include can be filtered out of the SameSpots Analysis
• Fully automatic SameSpots Analysis produces data with 100% matching and no missing values which allows robust statistical analysis

View Results and Identify Interesting Spots

• Significant spots are ranked for review by fold change and ANOVA (p-value)
• Each spot can be assessed using the 2D/3D montage view and expression profiles
• Any spot editing is greatly simplified as a single edit on one image and is automatically applied to all images across the entire experiment
• Interesting spots are highlighted on the image view as a guide for manual picking

Report Generation

• A simple, printable report provides an overview of the spots which warrant further investigation and are selected for picking

Software Flexibility

The Prodigy SameSpots software also allows the user to add images to an existing analyzed experiment. This improves the scope of proteomics studies as users are not confined to the original experiment design.

  2D Imaging with the UVP Biospectrum® 800 System