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iBox® Explorer TM 2 Imaging Microscope

Macro to Micro Fluorescent In Vivo Small Animal Imaging

Now researchers can take detection of fluorescent markers in small animals to a new level with the iBox Explorer 2 Imaging Microscope! The iBox Explorer 2 is unique in its ability to view macro to micro in the whole animal to individual cell, subcutaneously and within the body cavity of mice. The upright optics provide an ultra long working distance and high numerical aperture (NA) for detailed fluorescent in vivo imaging.

The iBox Explorer easily detects GFP/RFP and other fluorescent markers in small animals. The capabilities of this in vivo small animal imaging system enables research studies in whole animal down to individual cells.

iBox Explorer Imaging System

Applications

  • Tumor shedding
  • Tumor/host margins and interactions
  • Hematogenous trafficking
  • Tumor angiogenesis
  • Tumor micro environment
  • Intralymphatic trafficking
  • Micro/macro metastases
  • Primary tumor growth
  • Biodistribution

Main Features

  • Magnification ranges of 0.17x - 16.5x enables easy transition from the macroscopic to the microscope scale
  • iBox Explorer 2 enables researchers to visualize micro injection of cancer cells in vivo
  • Ability to image organs and cells subcutaneously and within the body cavity of living mice
  • Optical configurations are parcentered and parfocal, allowing seamless imaging through the magnification ranges
  • Leading-edge high frame rate cooled color camera enables quick detection, image capture and high throughput
  • Easy to use software automates research with templates for reproducible, consistent results
  • Bright illumination of samples produces an intense fluorescent signal and fast exposure times
  • Unlimited selection of filters enables users to image in the fluorescent, visible and NIR ranges for multiple applications (RFP/GFP included, others optional)
  • Generate accurate data with VisionWorks Software

iBox® Explorer 2 Imaging Microscope is sold under license from AntiCancer, Inc. allowing scientists to optimize their research using an extensive array of patented fluorescent protein imaging applications technologies.

Focusing on Fluorescent Cancer Cell Detection

In research performed, HT1080 fluorescent cancer cells were injected into the epigastrica cranialis. Immediately after the injection, the fluorescence indicates the cells escaped around the injection sites. In the zoomed in views, a cluster of cancer cells can be observed in the blood stream.

System Capabilities

  • Capture images with a superior cooled CCD camera and optics that are optimized for VIS - NIR imaging
  • View the whole animal to the single cell level via the motorized optics
  • Seamlessly navigate the image through all magnification ranges from 0.17x to 16.5x
  • Control BioLite excitation filters and light intensity via the software interface
  • Generate continuous excitation spectrum with the configurable filters for fluorescence microscopy
  • Slide-out warming plate maintains uniform temperature
  • Create optimum imaging conditions with the light tight darkroom
  • See quick sample inspection with unique viewing window
  • Simply adjust the position of the motorized X, Y, Z stage with the joystick

BioLite Xe MultiSpectral Light Source

  • Easy installation of the BioLite Xe external light source through the darkroom access ports
  • Epi fiber optic Xenon light source supplies uniform and directed lighting to the animal
  • Matched excitation/emission green fluorescent protein (GFP), red fluorescent protein (RFP) sets included; custom filter sets available
  • Eight excitation filter positions accommodate a variety of filters for specific applications
  • Six intensity settings from 0 to 100% relative intensity regulate the amount of illumination supplied through the fiber optic cable
Application Biolite Excitation
Wavelength
Microscope Emission
Wavelength
GFP 455-495nm 503-523nm
RFP 502-547nm 580-630nm
General   515 long pass
Neutral Density   400-700nm

Emission filters are placed in the darkroom filter tray. The neutral density filter passes emission wavelengths from 400-700nm. This filter is used for white light or non-fluorescent imaging. The long pass filter blocks blue light excitation and passes fluorescence.

Additional standard and custom filter sets are available to cover a wide spectra range of fluorescent proteins. For an extensive list filters available, go to Filters.

VisionWorks® Image Acquisition and Analysis Software

VisionWorks Image Acquisition and Analysis Software can be configured with the system or ordered separately. Check with your local representative for details. This sophisticated package provides comprehensive tools for image capture, enhancement and analysis. Capabilities include:

  • Image acquisition functions for simple image capture and expanded integration options
  • Estimate tumor volume
  • Measure linear dimensions of tumors
  • Apply numerous image enhancements to images
  • Comprehensive tools for advanced analysis including area density
  • Support for 21 CFR Part 11 compliance
  • One-touch automated macros
  • User defined templates, profiles and preferences
  • Generate extensive reports and export data
  • Multiple user network licenses available
Anesthesia Unit
  • Optional anesthesia unit for small animals produces a state of immobilization that allows acquisition of repeatable and reliable data

Support Resources

Manual

Technical support assistance and software drivers

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