VisionWorks®LS Analysis Software

• Image Acquisition Controls
• Advanced Analysis Tools
• Fluorescence Calibration Instrument
• One-Touch Macros
• Support for 21 CFR Part 11 Compliance
• Extensive Image Enhancement Capabilities
• User Defined Profiles and Preferences
• Detailed Report Generation

Image Acquisition

The VisionWorks®LS image capture function is configured to work with UVP’s BioImaging Systems. Capture images of any fluorescently labeled or colorimetric stained gels, blots, and membranes. For chemiluminescent imaging, acquire chemiluminescent Western, Northern and Southern blot images without using radioisotope labeled 32P and film. The user friendly software guides you through steps for capture of images. Integration options:

• On-chip integration for the simplest image capture; convert “live image preview” to captured image with one keystroke
• Sequential integration for automatic sequence of images of increasing exposure to ensure captured picture with optional exposure times; features automatic Digital Video Playback (DVP) of acquired images
• Dynamic integration for special sequential capture of Western Blots; each image is an addition of previous exposures so that an optimal picture can be formed in the minimum time before the chemiluminescence fades
   

Saturation Warning
Assure imaging results are quantifiable by using the saturation warning to to detect saturation of bands in live preview.

1D Lane Densitometry/Analysis

VisionWorks®LS analysis software includes comprehensive tools to provide a full range of analysis capabilities. VisionWorks®LS enables image acquisition and analysis for a wide range of applications including chemiluminescence, chemifluorescence, fluorescence, bioluminescence, colorimetric, and live animal samples. The easy to use functions automate your experiment analysis with accurate quantitation, generation of lane profile graphs, plus intensity histograms, concentration curves and much more!

Applications: (click links for screen images)

• Protein Quantitation
• Quantitative PCR
• cDNA Differential Expression
• GFP Expression Analysis
• FISH Analysis
• Gel Scoring

• PCR Gene Expression
• Colony Counting
• TLC Analysis
• Dot Blot Analysis
• Live Animal Imaging
• and more!
   

Maximizing Fluorescence Calibration

New! Using a non-photobleaching fluorescent standard step tablet placed on a FirstLight UV Illuminator, users can normalize the control and treatment regions (bands and spots) on acquired images again one another for varioution in lighting and optical conditions.  Images taken with the steptab can be analyzed with sophisticated statistics including mean and standard deviation, modified entropy and volume.
 

One-Touch Automated Macros

Create your own macros to automate time consuming routine procedures involving image capture, enhancement, analysis and data archiving. Assign a function key to recorded macros for one-touch automation.

Support for 21 CFR Part 11 Compliance

VisionWorks®LS Software supports image security and change logs to assist with 21 CFR Part 11 compliance.

• Track image and analysis history and show image properties and user comments
• Control of account set-up by Administrator, determine usernames and passwords, as well as permissions for viewing and editing images
• Maintain login history of all accounts
   

Image Enhancement Capabilities

VisionWorks®LS offers many image enhancement features, process filters and annotation capabilities as non destructive tools for visualization and publication (click links for screen images).

• Enhance images with brightness, contrast, gamma, emboss, invert and pseudo color
• Annotate with text, lines, arrows, ellipse, and highlighter tools; show, hide or burn into image
• Select from filters to sharpen, blur and remove frequency based noise, 3D Image Profiler
   

User Profiles and Preferences

Researchers can personalize their workspace by placing tool bar icons along the side or across the top, with or without text. Then save the profiles by user name. Set up user accounts with passwords to protect user data. Tool bar layout preferences can be saved for different users.

Profile Menu
For set up of multiple users
and experiment preferences.

Report Generation

• Save images as various file formats including TIFF, JPEG, BMP, TGA, PNG and GIF for use in presentations or documentation
• Create detailed and user-configured reports showing extensive analysis results on MW, Rf, precise position of bands, band intensities, etc.
• Use the Filter Data button to choose specific data for reports
• Configure the Qwik-Link icon to automatically open an external software application
• Export data to Microsoft Excel or simple-text using Dynamic Data Exchange (DDE)